Lost Tiffany Necklace!

I'm sorry to be asking of this, since I know there are greater things to be worried with, but I come asking for direction and assistance. My boyfriend bought me a necklace from Tiffany's for Christmas, and I misplaced it on New Years Eve (or so I think). We spent the night at a motel, but the cleaning lady insists the necklace wasn't there when she cleaned up. I borrowed a duffel bag for the night from my mother, but emptied the bag completely before giving it back to her. While at the motel, I remember putting the necklace in the bag before bed, but when I emptied the bag a few days later, the necklace was gone. I've searched my room, but I can't find the necklace anywhere. I keep getting the feeling that it's in my house still, but I can't seem to place where. Please help if you have the time, thank you so much.