PCR tests. How they "work" and what you aren't being told.

"A typical amplification reaction includes target DNA, a thermostable DNA polymerase, two oligonucleotide primers, deoxynucleotide triphosphates (dNTPs), reaction buffer and magnesium. Once assembled, the reaction is placed in a thermal cycler, an instrument that subjects the reaction to a series of different temperatures for set amounts of time. This series of temperature and time adjustments is referred to as one cycle of amplification. Each PCR cycle theoretically doubles the amount of targeted sequence (amplicon) in the reaction. Ten cycles theoretically multiply the amplicon by a factor of about one thousand; 20 cycles, by a factor of more than a million in a matter of hours."

[link to www.promega.com (secure)]



[link to www.youtube.com (secure)]

"Typically, a PCR is a three-step reaction. The sample containing a dilute concentration of template DNA is mixed with a heat-stable DNA polymerase, such as Taq polymerase, primers, deoxynucleoside triphosphates (dNTPs), and magnesium. In the first step of PCR, the sample is heated to 95–98°C, which denatures the double-stranded DNA, splitting it into two single strands. In the second step, the temperature is decreased to approximately 55–65°C, allowing the primers to bind, or anneal, to specific sequences of DNA at each end of the target sequence, also known as the template. In the third step, the temperature is typically increased to 72°C, allowing the DNA polymerase to extend the primers by the addition of dNTPs to create a new strand of DNA, thus doubling the quantity of DNA in the reaction. This sequence of denaturation, annealing, and extension is repeated for many cycles, resulting in the exponential amplification of the template DNA. As the DNA polymerase loses activity or the dNTPs and primers are consumed, the reaction rate reaches a plateau."
[link to www.bio-rad.com (secure)]

I didn't ask you to care! As I wrote, I saw people posting opinion and belief and I put my 2 cents in. Are your 2 cents worth more than mine?!

Again, keep or toss the information.

I don't expect a single person on this site to go to reputable sources of information!

I never claimed to have the facts, personally! I have just pointed out that experts disagree.

You disagree with experts in this...good for you! You must be proud!

I didn't NEED to be "pepared"! I was just saying, hey, there us thus guy who sees things differently...and you get upset.

You're fucking crazy. Bye

Dont be an idiot, you think you are being funny but you arent

look I know shit about medicine or medical science but humor me here...

why does every med commercial have such a long list of complications attached if everything in the med world is A-okay?

Kary Mullis disagrees.

I'm guessin doc walton knows alot more about coronavirus testing than your stupid ass. you seem like a fuckin commie tard! go eat shit cumbucket!

Link? I know the top surgeon (drinking/footy buddy) here...nada deaths from Covid in a year. Show me your data.

We all sing off the same songsheet

Thread: Listen People, If You're Getting a PCR Test for Covid, You Need to Ask For Your CT Value...NO EXCUSES!!
Thread: The COVID-19 RT-PCR Test: How to Mislead All Humanity. Using a “Test” To Lock Down Society

Bogus test...bogus scamdemic. The people (supposedly) that buy the lie are too afraid of looking like the morans they are so they cling to attachments.

If that is true it is because people are sickening themselves with masks.

Fuck you, little cunt

Whassup, Doc?! So glad you're weighin' in on this thread.


And yeah, the Old North State is so much worse than people would believe when it comes to corruption. I know first hand and am anxiously munching my popcorn to see how the grand-poobahs runnin' my state into the ground will be dealt with soon.

The only way to get an accurate answer to your question, would be to actually parse the differences between a true cvo2 patient, a presumed cov2 patient, and a patient who's ill due to completely unrelated cov2 issues, yet had a "positive" test with a CT value of 30+. Tragically, the last two categories are currently amplified due to lockdowns themselves.

Last Edited by Dr. Van Gogh on 11/25/2020 12:31 PM

Needs to be taken out back with a shotgun - full disclosure I have never owned nor have I handled a firearm nor do I intend on obtaining one in the near future

Hello and ty! Sadly, I have no high expectations for Cooper whatsoever. He's abhorrent beyond measure.

The left at their games again.. their sources better than your sources.. their views are gospel and your complaints and doubts are ravings of the fantastically insane..

A yes or no test can never be 0% accurate... It can however be 0% reliable.

Can you imagine a person saying that they support your right to wear a mask, but come near me with a mask on and you will "pay the price".

It will probably get there as more people wake up and realize that the masktards are the problem and the idiotic tools of the government.

OP, I have worked with PCR for 15 years and can tell you it is extremely accurate and the cycle issue you described makes no sense.


I run 33-37 cycles all the time. That allows me to amplify a very small amount virus or other DNA target to a large enough amount to work with.


So how do I know I am right?


I run a negative and positive control with every run. If those are not accurate the whole run is tossed.


Second, if I have ten specimens and 2 are positive, I will rerun the positive specimens again to confirm.


There is no way the technology could be "tricking me" because there are to many safeguards with positive and negative controls plus repeating positive samples.

Saying "It's a PCR test so it's good." or "It's a PCR test so it's not good." is like saying "We used purified water in the test, so it's good (or not good)."

PCR can be associated with good tests or bad tests. It's just a testing method that's sometimes useful and sometimes not.

So you're concerned about false positives and you rerun them again to confirm. That's prudent.

Positives aren't being rerun with Covid tests, are they?

Last Edited by oniongrass on 11/25/2020 01:21 PM

Let me elaborate further. You can't get a false positive for no reason, meaning the technology can't just invent a positive out of thin air.


However it is so sensitive, that false positives are usually cross contamination. Let's say a nurse handed the specimen before sending and had a tiny amount of the virus on her glove. If that made it into the run from grabbing the outside of the tube or something it will pick it up.

but how do you know the positive indicates the supposed virus?

For whatever unknown reasons, false positives occur. The same risk exists with Covid tests.

Good question, because PCR is only amplifying a DNA fragment that is an exact match..like a line of code...for that virus.


The positive control has that exact line of code in it. The negative is DNA without it.

Look guys, I am a scientist and conspiracy buff.


I do think it escaped a Wuhan Lab and they were probably doing something called "Gain of Function" research. That is when scientists in their grand stupidity make something easier to spread to study how it works (I do not agree with this type of work).



The smoking gun here is we got nailed with an escaped lab engineered bug and the govt would rather you think it's fake than the other.

how do you know that sequence is unique to the supposed virus? how do you know what sequence to look for?