Want to read a research paper from 2017 involving vaccines, engineered human cells, gene-encoded proteins, and kill switches?

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Want to read a research paper from 2017 involving vaccines, engineered human cells, gene-encoded proteins, and kill switches?
Anonymous Coward User ID: 80154986 United States 08/12/2021 02:48 PM Report Abusive Post Report Copyright Violation	Want to read a research paper from 2017 involving vaccines, engineered human cells, gene-encoded proteins, and kill switches? Well, here you go. ---------- A Strategy for Creating Organisms Dependent on Noncanonical Amino Acids Dr. Weimin Xuan, Prof. Peter G. Schultz First published: 07 June 2017 The use of noncanonical amino acids (ncAAs) to control the viability of an organism provides a strategy for the development of conditional “kill switches” for live vaccines or engineered human cells. We report an approach inspired by the posttranslational acetylation/deacetylation of lysine residues, in which a protein encoded by a gene with an in-frame nonsense codon at an essential lysine can be expressed in its native state only upon genetic incorporation of N-E-acetyl-l-Lys (AcK), and subsequent enzymatic deacetylation in the host cell. We applied this strategy to two essential E. coli enzymes: the branched-chain aminotransferase BCAT and the DNA replication initiator protein DnaA. We also devised a barnase-based conditional suicide switch to further lower the escape frequency of the host cells. This strategy offers a number of attractive features for controlling host viability, including a single small-molecule-based kill switch, low escape frequency, and unaffected protein function. [link to doi.org (secure)]

Anonymous Coward (OP) User ID: 80154986 United States 08/12/2021 03:32 PM Report Abusive Post Report Copyright Violation	Re: Want to read a research paper from 2017 involving vaccines, engineered human cells, gene-encoded proteins, and kill switches? Unfortunately the full paper is behind a paywall.
Anonymous Coward (OP) User ID: 80154986 United States 08/12/2021 03:36 PM Report Abusive Post Report Copyright Violation	Re: Want to read a research paper from 2017 involving vaccines, engineered human cells, gene-encoded proteins, and kill switches? Here are some others to look into. Stimuli-Responsive DNA-Functionalized Nano-/Microcontainers for Switchable and Controlled Release [link to onlinelibrary.wiley.com (secure)] Mechanically Interlocked Molecules (MIMs)—Molecular Shuttles, Switches, and Machines (Nobel Lecture) [link to doi.org (secure)]
Anonymous Coward (OP) User ID: 80154986 United States 08/13/2021 01:26 AM Report Abusive Post Report Copyright Violation	Re: Want to read a research paper from 2017 involving vaccines, engineered human cells, gene-encoded proteins, and kill switches? :bump9:

Anonymous Coward (OP) User ID: 80154986 United States 08/13/2021 01:35 AM Report Abusive Post Report Copyright Violation	Re: Want to read a research paper from 2017 involving vaccines, engineered human cells, gene-encoded proteins, and kill switches? Thread: Engineering a genetically encoded magnetic protein crystal
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