DO YOU ASSHOLES REALIZE THEY CANNOT DIAGNOSE COVID-19 without growing a culture in a petri dish. CoVid-21? | |
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Jake
User ID: 77849624 United States 03/14/2020 08:41 PM Report Abusive Post Report Copyright Violation | Re: DO YOU ASSHOLES REALIZE THEY CANNOT DIAGNOSE COVID-19 without growing a culture in a petri dish. CoVid-21? You cannot possibly be that dumb Evil controls the ignorant... Climate change is a hoax so is the vax you have been fear-porned into compliance! Definition Satan from the bible: Satan (Rev 12:7) exercising his subtle (indirect) impact on heathen governments (powers) – i.e. accomplishing his hellish agenda from "behind the scenes." |
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Q33
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Anonymous Coward (OP) User ID: 66280474 Czechia 03/14/2020 09:11 PM Report Abusive Post Report Copyright Violation | Re: DO YOU ASSHOLES REALIZE THEY CANNOT DIAGNOSE COVID-19 without growing a culture in a petri dish. CoVid-21? Comparison of high-throughput sequencing methods[73][74] Method Read length Accuracy (single read not consensus) Reads per run Time per run Cost per 1 billion bases (in US$) Advantages Disadvantages Single-molecule real-time sequencing (Pacific Biosciences) 30,000 bp (N50); maximum read length >100,000 bases[75][76][77] 87% raw-read accuracy[78] 4,000,000 per Sequel 2 SMRT cell, 100–200 gigabases[75][79][80] 30 minutes to 20 hours[75][81] $7.2-$43.3 Fast. Detects 4mC, 5mC, 6mA.[82] Moderate throughput. Equipment can be very expensive. Ion semiconductor (Ion Torrent sequencing) up to 600 bp[83] 99.6%[84] up to 80 million 2 hours $66.8-$950 Less expensive equipment. Fast. Homopolymer errors. Pyrosequencing (454) 700 bp 99.9% 1 million 24 hours $10,000 Long read size. Fast. Runs are expensive. Homopolymer errors. Sequencing by synthesis (Illumina) MiniSeq, NextSeq: 75–300 bp; MiSeq: 50–600 bp; HiSeq 2500: 50–500 bp; HiSeq 3/4000: 50–300 bp; HiSeq X: 300 bp 99.9% (Phred30) MiniSeq/MiSeq: 1–25 Million; NextSeq: 130-00 Million; HiSeq 2500: 300 million – 2 billion; HiSeq 3/4000 2.5 billion; HiSeq X: 3 billion 1 to 11 days, depending upon sequencer and specified read length[85] $5 to $150 Potential for high sequence yield, depending upon sequencer model and desired application. Equipment can be very expensive. Requires high concentrations of DNA. Combinatorial probe anchor synthesis (cPAS- BGI/MGI) BGISEQ-50: 35-50bp; MGISEQ 200: 50-200bp; BGISEQ-500, MGISEQ-2000: 50-300bp[86] 99.9% (Phred30) BGISEQ-50: 160M; MGISEQ 200: 300M; BGISEQ-500: 1300M per flow cell; MGISEQ-2000: 375M FCS flow cell, 1500M FCL flow cell per flow cell. 1 to 9 days depending on instrument, read length and number of flow cells run at a time. $5- $120 Sequencing by ligation (SOLiD sequencing) 50+35 or 50+50 bp 99.9% 1.2 to 1.4 billion 1 to 2 weeks $60-130 Low cost per base. Slower than other methods. Has issues sequencing palindromic sequences.[87] Nanopore Sequencing Dependent on library preparation, not the device, so user chooses read length (up to 2,272,580 bp reported[88]). ~92–97% single read dependent on read length selected by user data streamed in real time. Choose 1 min to 48 hrs $7-100 Longest individual reads. Accessible user community. Portable (Palm sized). Lower throughput than other machines, Single read accuracy in 90s. GenapSys Sequencing Around 150 bp single-end 99.9% (Phred30) 1 to 16 million Around 24 hours $667 Low-cost of instrument ($10,000) Chain termination (Sanger sequencing) 400 to 900 bp 99.9% N/A 20 minutes to 3 hours $2,400,000 Useful for many applications. More expensive and impractical for larger sequencing projects. This method also requires the time-consuming step of plasmid cloning or PCR. |
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Anonymous Coward User ID: 78613509 Canada 03/14/2020 09:16 PM Report Abusive Post Report Copyright Violation | Re: DO YOU ASSHOLES REALIZE THEY CANNOT DIAGNOSE COVID-19 without growing a culture in a petri dish. CoVid-21? they took a swab from Q33's little tiny sized micro-brain & the test results said it was 100% pure yeast infection now they use that stanky infected swab to ward off all the evil in the world |
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Anonymous Coward User ID: 78613509 Canada 03/14/2020 09:47 PM Report Abusive Post Report Copyright Violation | Re: DO YOU ASSHOLES REALIZE THEY CANNOT DIAGNOSE COVID-19 without growing a culture in a petri dish. CoVid-21? they took a swab from Q33's little tiny sized micro-brain & the test results said it was 100% pure yeast infection Quoting: Anonymous Coward 78613509 now they use that stanky infected swab to ward off all the evil in the world Vag infection? yup growing on his brain its remarkable, because Q33 doesnt even like pussy. He dreams about cock 24/7 |
Q33
User ID: 27169207 Canada 03/14/2020 09:48 PM Report Abusive Post Report Copyright Violation | Re: DO YOU ASSHOLES REALIZE THEY CANNOT DIAGNOSE COVID-19 without growing a culture in a petri dish. CoVid-21? they took a swab from Q33's little tiny sized micro-brain & the test results said it was 100% pure yeast infection Quoting: Anonymous Coward 78613509 now they use that stanky infected swab to ward off all the evil in the world Vag infection? yup growing on his brain its remarkable, because Q33 doesnt even like pussy. He dreams about cock 24/7 Facts! |
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Anonymous Coward User ID: 78422833 United States 03/14/2020 09:50 PM Report Abusive Post Report Copyright Violation | Re: DO YOU ASSHOLES REALIZE THEY CANNOT DIAGNOSE COVID-19 without growing a culture in a petri dish. CoVid-21? they took a swab from Q33's little tiny sized micro-brain & the test results said it was 100% pure yeast infection Quoting: Anonymous Coward 78613509 now they use that stanky infected swab to ward off all the evil in the world Vag infection? yup growing on his brain its remarkable, because Q33 doesnt even like pussy. He dreams about cock 24/7 Is that specifically HEBREW cock you speak of? |
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Q33
User ID: 27169207 Canada 03/14/2020 10:01 PM Report Abusive Post Report Copyright Violation | Re: DO YOU ASSHOLES REALIZE THEY CANNOT DIAGNOSE COVID-19 without growing a culture in a petri dish. CoVid-21? yup growing on his brain its remarkable, because Q33 doesnt even like pussy. He dreams about cock 24/7 Is that specifically HEBREW cock you speak of? I dont think Q33 cares any old cock will do when it comes to cocks Q33 is like a kid in a candy store Projecting again eh? |
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